Isolation and partial characterization of antimicrobial proteins /peptides from Moringa oleifera

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Anum Shakir Sadaf Riaz Muhammad Afzal, Mr. Basit Zeshan Salma Batool

Abstract

Moringa oleifera is worldwide distributed plant because of its number of pharmacological and nutritional benefits. In this study, protein extracts of M. oleifera leaves and flowers were prepared. Extracts were purified by dialysis and quality of the sample was assessed through SDS-PAGE. Antimicrobial and antioxidant activity of these extracts was determined by using of disk diffusion method, Dpph (free radical scavenging assay) and enzymatic assay. The antimicrobial activity of M. oleifera protein extract of flowers and leaves were determined against multidrug – resistant bacteria e.g Methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli and Salmonella typhi. Extract results showed the maximum zone of inhibition against MRSA and minimum zone of inhibition against E. coli and S. typhi whereas protein extract of leaves did not show any antibacterial activity against these bacteria. MIC of flower extract was determined as 166ug/ml against MRSA whereas E. coli and S. typhi was recorded as 500ug/ml. enzymatic activity (peroxidase) result showed that leaf and flower protein extract possess higher enzymatic activity at time of 10 minutes and lower at time of 20 and 30 minutes. Protein extract of leaf showed higher scavenging activity at time of 40and 60 and lower at time of 20 whereas flower protein extract showed higher scavenging activity at time of 60 min and lower at time of 40 and 20. Present study showed that M. oleifera possess potent antimicrobial and antioxidant activity & it may be used as potential drug against multidrug- resistant bacteria.   

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